M20 Seq

Most single-cell RNA sequencing technologies rely on poly(A) tails to capture RNA, which only captures the 3' or 5' end of mRNA. In contrast, M20 Seq captures full-length transcriptomes by leveraging the power of random primers. In addition to analyzing gene expression, this unique methodology enables the single-cell analysis of non-coding RNA, alternative splicing, gene fusions, copy number variations, gene mutations and other features.

M20 Seq eliminates the requirement for high cell viability, enabling its use with a variety of sample types such as fresh, frozen, and formalin-fixed paraffin-embedded (FFPE) samples. This capability surpasses typical limitations of most single-cell RNA sequencing technologies related to sample quality, processing time, and geographic constraints, rendering it ideal for centralized laboratories.

The random primer-based RNA capture approach expands M20 Seq applicability to a wide array of species, covering both eukaryotic organisms—including humans, animals and plants—and prokaryotic organisms like bacteria.

M20 Seq technology significantly increases cell capture, scaling capabilities to the millions.

Utilizing the power of random primers, M20 Seq facilitates multiple capture opportunities for each RNA molecule, significantly enhancing gene detection capabilities.

M20 Seq has demonstrated excellent detection results on both droplet-based and microwell-based platforms.