The High-Throughput Single-Cell Transcriptome for Frozen Samples provides a comprehensive solution for detecting gene expression in eukaryotic cryopreserved samples. By seamlessly integrating the innovative VITApilote High-Throughput Single-Cell Transcriptome Kit for Frozen Samples, alongside the high-performance VITAcruizer Single-Cell Partitioning System and the advanced VITAseer Bioinformatics Software, this platform enables unbiased full-length transcriptome profiling. With the capacity to analyze 1,000-20,000 cells per sample, it facilitates a thorough investigation of mRNA, non-coding RNA, alternative splicing, and more.